Recombinant Antibody Selections by Combining Phage and Yeast Display.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8958-4_16
Abstract: In vitro display technologies have put together the generation of large antibody libraries with selection and screening procedures to identify lead candidates. Phage display antibody libraries allow selecting and identifying binders for a variety of… read more here.
Keywords: phage yeast; antibody; display; yeast display ... See more keywords
Methods for Construction of Yeast Display Libraries of Four-Domain T-Cell Receptors.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_13
Abstract: Since two decades, yeast display methodology is a popular tool for discovery, stability improvement, and affinity maturation of diverse protein scaffolds, intended for antigen recognition. Yeast display is particularly well suited for the selection of… read more here.
Keywords: methods construction; yeast display; display; cell receptors ... See more keywords
Yeast Display Enables Identification of Covalent Single-Domain Antibodies against Botulinum Neurotoxin Light Chain A.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00574
Abstract: While covalent drug discovery is reemerging as an important route to small-molecule therapeutic leads, strategies for the discovery and engineering of protein-based irreversible binding agents remain limited. Here, we describe the use of yeast display… read more here.
Keywords: yeast display; display; single domain; neurotoxin light ... See more keywords
Repertoire-scale determination of class II MHC peptide binding via yeast display improves antigen prediction
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-18204-2
Abstract: CD4+ helper T cells contribute important functions to the immune response during pathogen infection and tumor formation by recognizing antigenic peptides presented by class II major histocompatibility complexes (MHC-II). While many computational algorithms for predicting… read more here.
Keywords: peptide binding; yeast display; prediction; mhc ... See more keywords
Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice
Sign Up to like & getrecommendations! Published in 2020 at "Microbial Cell Factories"
DOI: 10.1186/s12934-020-01316-1
Abstract: Background Existing methods for preparing influenza vaccines pose the greatest challenge against highly pandemic avian influenza H7N9 outbreak in the poultry and humans. Exploring a new strategy for manufacturing and delivering a safe and effective… read more here.
Keywords: yeast display; challenge; oral vaccine; h7n9 ... See more keywords
Direct selection of functional fluorescent-protein antibody fusions by yeast display
Sign Up to like & getrecommendations! Published in 2023 at "PLOS ONE"
DOI: 10.1371/journal.pone.0280930
Abstract: Antibodies are important reagents for research, diagnostics, and therapeutics. Many examples of chimeric proteins combining the specific target recognition of antibodies with complementing functionalities such as fluorescence, toxicity or enzymatic activity have been described. However,… read more here.
Keywords: direct selection; functional fluorescent; yeast display; protein ... See more keywords
Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22041634
Abstract: We present the construction and screening of yeast display libraries of post-translationally modified peptides wherein site-selective enzymatic treatment of linear peptides is achieved using bacterial transglutaminase. To this end, we developed two alternative routes, namely… read more here.
Keywords: peptide; yeast display; display libraries; treated peptides ... See more keywords
A high-throughput yeast display approach to profile pathogen proteomes for MHC-II binding
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.78589
Abstract: T cells play a critical role in the adaptive immune response, recognizing peptide antigens presented on the cell surface by Major Histocompatibility Complex (MHC) proteins. While assessing peptides for MHC binding is an important component… read more here.
Keywords: mhc binding; yeast display; high throughput; approach ... See more keywords