Genome-Wide Analysis of Mitotic Recombination in Budding Yeast.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0644-5_15
Abstract: DNA break lesions pose a serious threat to the integrity of the genome. Eukaryotic cells can repair these lesions using the homologous recombination pathway that guides the repair reaction by using a homologous DNA template.… read more here.
Keywords: budding yeast; genome wide; yeast genome; analysis ... See more keywords
ACtivE: Assembly and CRISPR-Targeted in Vivo Editing for Yeast Genome Engineering Using Minimum Reagents and Time
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00175
Abstract: Among the numerous genetic tools developed for yeast, CRISPR/Cas system has been a widely used genome editing method thanks to its sophistication. However, CRISPR methods for yeast generally rely on pre-assembled DNAs and extra cloning… read more here.
Keywords: dna; crispr; integration; yeast genome ... See more keywords
Discovering and genotyping genomic structural variations by yeast genome synthesis and inducible evolution.
Sign Up to like & getrecommendations! Published in 2020 at "FEMS yeast research"
DOI: 10.1093/femsyr/foaa012
Abstract: Genomic structural variations (SVs) promote the evolution of Saccharomyces cerevisiae, and play an important role in phenotypic diversities. Yeast genomic structures can be remodeled by design and bottom-up synthesis. The synthesis of yeast genome creates… read more here.
Keywords: genomic structural; structural variations; yeast genome; inducible evolution ... See more keywords
From Petri Plates to Petri Nets, a revolution in yeast biology
Sign Up to like & getrecommendations! Published in 2022 at "FEMS Yeast Research"
DOI: 10.1093/femsyr/foac008
Abstract: In the beginning was... the genome. The 30th of January, 1991 found me outside Temple Underground Station on the Thames Embankment in London. John Sgouros of the Martinsried Institute for Protein Sciences, who had flown… read more here.
Keywords: yeast genome; sequence; chromosome; biology ... See more keywords
Shuffling the yeast genome using CRISPR/Cas9-generated DSBs that target the transposable Ty1 elements
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DOI: 10.1371/journal.pgen.1010590
Abstract: Although homologous recombination between transposable elements can drive genomic evolution in yeast by facilitating chromosomal rearrangements, the details of the underlying mechanisms are not fully clarified. In the genome of the yeast Saccharomyces cerevisiae, the… read more here.
Keywords: recombination; yeast genome; cas9; shuffling yeast ... See more keywords
A practical comparison of the next-generation sequencing platform and assemblers using yeast genome
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DOI: 10.26508/lsa.202201744
Abstract: This research analyzes the general and optimal de novo yeast assembly pipeline using the repetitive yeast genome assembly by benchmarking four different sequencing platforms and seven assembly programs. Assembling fragmented whole-genomic information from the sequencing… read more here.
Keywords: comparison; yeast genome; sequencing platforms; generation sequencing ... See more keywords