Humanization of Chicken‐Derived scFv Using Yeast Surface Display and NGS Data Mining
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DOI: 10.1002/biot.202000231
Abstract: Generation of high‐affinity monoclonal antibodies by immunization of chickens is a valuable strategy, particularly for obtaining antibodies directed against epitopes that are conserved in mammals. A generic procedure is established for the humanization of chicken‐derived… read more here.
Keywords: affinity; yeast surface; chicken; humanization ... See more keywords
Directed Evolution of Protein Thermal Stability Using Yeast Surface Display.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6857-2_4
Abstract: Yeast surface display is a powerful protein engineering technology that has been used for many applications including engineering protein stability. Direct screening for improved thermal stability can be accomplished by heat shock of yeast displayed… read more here.
Keywords: protein; engineering; yeast surface; stability ... See more keywords
Isolation of Antigen-Specific VHH Single-Domain Antibodies by Combining Animal Immunization with Yeast Surface Display.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_10
Abstract: In addition to conventional hetero-tetrameric antibodies, the adaptive immune repertoire of camelids comprises the so-called heavy chain-only antibodies devoid of light chains. Consequently, antigen binding is mediated solely by the variable domain of the heavy… read more here.
Keywords: isolation antigen; yeast surface; antigen specific; single domain ... See more keywords
Rapid Generation of Chicken Immune Libraries for Yeast Surface Display.
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DOI: 10.1007/978-1-4939-9853-1_16
Abstract: Fluorescence-activated cell sorting (FACS) in combination with yeast surface display has emerged as a vital tool for the isolation and engineering of antibodies and antibody-derived fragments from synthetic, naïve, and immune libraries. However, the generation… read more here.
Keywords: generation; surface display; immune libraries; yeast surface ... See more keywords
Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian Cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.794389
Abstract: Yeast-surface display (YSD) is commonly applied to screen Fab immune or naïve libraries for binders of predefined target molecules. However, reformatting of isolated variants represents a time-intensive bottleneck. Herein, we present a novel approach to… read more here.
Keywords: yeast surface; surface display; immune libraries; antibody ... See more keywords
Yeast Surface Display Platform for Rapid Selection of an Antibody Library via Sequential Counter Antigen Flow Cytometry
Sign Up to like & getrecommendations! Published in 2022 at "Antibodies"
DOI: 10.3390/antib11040061
Abstract: Yeast surface display techniques have been increasingly employed as a tool for both the discovery and affinity maturation of antibodies. In this study, we describe the use of yeast surface display for the selection and… read more here.
Keywords: yeast surface; scfv; selection; surface display ... See more keywords