The Yeast Three-Hybrid System for Protein Interactions.
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DOI: 10.1007/978-1-4939-7871-7_12
Abstract: Proteins rarely act alone as their functions tend to be regulated in vivo. Therefore, protein-protein interaction analyses provide key clues for understanding the complex biological processes in the living cell. Several techniques have been developed… read more here.
Keywords: protein; hybrid system; three hybrid; protein interactions ... See more keywords
The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants.
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DOI: 10.1007/978-1-4939-7871-7_13
Abstract: Yeast-hybrid methods have been successfully applied for screening interacting partners of DNAs or proteins. A yeast-based method, the yeast three-hybrid system, using a chimeric protein of a DNA-binding domain (LexA or GAL4BD) with a protein… read more here.
Keywords: hybrid system; three hybrid; binding proteins; rna binding ... See more keywords
Using the yeast three-hybrid system for the identification of small molecule-protein interactions with the example of ethinylestradiol
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DOI: 10.1515/hsz-2021-0355
Abstract: Abstract Since the first report on a yeast three-hybrid system, several approaches have successfully utilized different setups for discovering targets of small molecule drugs. Compared to broadly applied MS based target identification approaches, the yeast… read more here.
Keywords: system; three hybrid; identification; yeast three ... See more keywords