Identification of PDZ Interactions by Yeast Two-Hybrid Technique.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1166-1_1
Abstract: The yeast two-hybrid technique is a powerful method to detect direct protein-protein interactions. Due to its accessibility, speed, and versatility, this technique is easy to set up in any laboratory and suitable for small and… read more here.
Keywords: technique; two hybrid; identification pdz; hybrid technique ... See more keywords
Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system
Sign Up to like & getrecommendations! Published in 2018 at "Parasitology Research"
DOI: 10.1007/s00436-018-5812-z
Abstract: Toxoplasma gondii deploys many effector proteins in order to hijack and manipulate host cell signaling pathways, allowing parasite colonization, subversion of immune responses, and disease progression. T. gondii effector protein 14-3-3 (Tg14-3-3) promotes parasite dissemination… read more here.
Keywords: protein; host; yeast two; toxoplasma gondii ... See more keywords
A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein–Protein Interactions
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00192
Abstract: The yeast two-hybrid (Y2H) assay is widely used for protein–protein interaction characterization due to its simplicity and accessibility. However, it may mask changes in affinity caused by mutations or ligand activation due to signal saturation.… read more here.
Keywords: protein protein; two hybrid; expression; protein interactions ... See more keywords
Directed Evolution of Herbicide Biosensors in a Fluorescence-Activated Cell-Sorting-Compatible Yeast Two-Hybrid Platform
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00297
Abstract: Developing sensory modules for specific molecules of interest represents a fundamental challenge in synthetic biology and its applications. A somewhat generalizable approach for this challenge is demonstrated here by evolving a naturally occurring chemically induced… read more here.
Keywords: two hybrid; hybrid platform; directed evolution; yeast two ... See more keywords
A yeast two-hybrid screening identifies novel Atg8a interactors in Drosophila
Sign Up to like & getrecommendations! Published in 2022 at "Autophagy"
DOI: 10.1080/15548627.2022.2045535
Abstract: ABSTRACT Macroautophagy/autophagy-related protein Atg8/LC3 is important for autophagosome biogenesis and required for selective degradation of various substrates. In our recent study, we performed a yeast two-hybrid screening to identify proteins that interact with Atg8a, the… read more here.
Keywords: hybrid screening; two hybrid; novel atg8a; screening identifies ... See more keywords
Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx1173
Abstract: Abstract Protein-protein interaction (PPI) network maintains proper function of all organisms. Simple high-throughput technologies are desperately needed to delineate the landscape of PPI networks. While recent state-of-the-art yeast two-hybrid (Y2H) systems improved screening efficiency, either… read more here.
Keywords: system; based library; two hybrid; recombination based ... See more keywords
Yeast two‐ and three‐species hybrids and high‐sugar fermentation
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DOI: 10.1111/1751-7915.13390
Abstract: The dominating strains of most sugar‐based natural and industrial fermentations either belong to Saccharomyces cerevisiae and Saccharomyces uvarum or are their chimeric derivatives. Osmotolerance is an essential trait of these strains for industrial applications in… read more here.
Keywords: three species; high sugar; yeast two; sugar ... See more keywords
Novel binding partners for Prenylated Rab Acceptor 1 identified by a split-ubiquitin yeast two-hybrid screen
Sign Up to like & getrecommendations! Published in 2019 at "BMC Research Notes"
DOI: 10.1186/s13104-019-4219-y
Abstract: ObjectivePrenylated Rab Acceptor 1 (PRA1) is a transmembrane protein localized to the early secretory pathway. It has been found to interact with an array of Rab GTPases, leading to its hypothesized function in the recycling… read more here.
Keywords: split ubiquitin; binding partners; two hybrid; screen ... See more keywords
A user-friendly platform for yeast two-hybrid library screening using next generation sequencing
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0201270
Abstract: Yeast two-hybrid (Y2H) is a well-established genetics-based system that uses yeast to selectively display binary protein-protein interactions (PPIs). To meet the current need to unravel complex PPI networks, several adaptations have been made to establish… read more here.
Keywords: library screening; two hybrid; method; yeast two ... See more keywords