An Integrated Phenotypic and Genotypic Approach Reveals a High‐Risk Subtype Association for EBF3 Missense Variants Affecting the Zinc Finger Domain
Sign Up to like & getrecommendations! Published in 2022 at "Annals of Neurology"
DOI: 10.1002/ana.26359
Abstract: Collier/Olf/EBF (COE) transcription factors have distinct expression patterns in the developing and mature nervous system. To date, a neurological disease association has been conclusively established for only the Early B‐cell Factor‐3 (EBF3) COE family member… read more here.
Keywords: missense variants; risk; finger domain; zinc finger ... See more keywords
Chemical Approach to Biological Safety: Molecular‐Level Control of an Integrated Zinc Finger Nuclease
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201700420
Abstract: Application of artificial nucleases (ANs) in genome editing is still hindered by their cytotoxicity related to off‐target cleavages. This problem can be targeted by regulation of the nuclease domain. Here, we provide an experimental survey… read more here.
Keywords: zinc finger; nuclease; integrated zinc; approach ... See more keywords
Epitope Fine Mapping by Mass Spectrometry: Investigations of Immune Complexes Consisting of Monoclonal Anti‐HpTGEKP Antibody and Zinc Finger Protein Linker Phospho‐Hexapeptides
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200390
Abstract: Accurate formation of antibody‐antigen complexes has been relied on in both, multitudes of scientific projects and ample therapeutic and diagnostic applications. Mass spectrometrically determined dissociation behavior of immune complexes with the anti‐HpTGEKP antibody revealed that… read more here.
Keywords: immune complexes; linker; phospho; zinc finger ... See more keywords
The zinc finger transcription factor Sall1 is required for the early developmental transition of microglia in mouse embryos
Sign Up to like & getrecommendations! Published in 2022 at "Glia"
DOI: 10.1002/glia.24192
Abstract: Microglia play many critical roles in neural development. Recent single-cell RNA-sequencing studies have found diversity of microglia both across different stages and within the same stage in the developing brain. However, how such diversity is… read more here.
Keywords: transition; finger transcription; transcription factor; factor sall1 ... See more keywords
Zinc finger E‐box binding homeobox 1 and atherosclerosis: New insights and therapeutic potential
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.30177
Abstract: Zinc finger E‐box binding homeobox 1 (ZEB1), an important transcription factor belonging to the ZEB family, plays a crucial role in regulating gene expression required for both normal physiological and pathological processes. Accumulating evidence has… read more here.
Keywords: zinc finger; finger box; box binding; binding homeobox ... See more keywords
Characterizing the role of the zinc finger transcription factor AcrpnR in governing development in Aspergillus cristatus.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of basic microbiology"
DOI: 10.1002/jobm.202100305
Abstract: Filamentous fungi reproduce sexually or asexually, and the developmental processes are strictly regulated by a variety of transcription factors. In this study, we characterized a zinc finger transcription factor, called AcrpnR, in Aspergillus cristatus (GME2916).… read more here.
Keywords: transcription; zinc finger; cristatus; transcription factor ... See more keywords
Deciphering metal ion preference and primary coordination sphere robustness of a designed zinc finger with high‐resolution mass spectrometry
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3067
Abstract: Small zinc finger (ZnF) motifs are promising molecular scaffolds for protein design owing to their structural robustness and versatility. Moreover, their characterization provides important insights into protein folding in general. ZnF motifs usually possess an… read more here.
Keywords: coordination; zinc; zinc finger; ion ... See more keywords
The unique N‐terminal zinc finger of synaptotagmin‐like protein 4 reveals FYVE structure
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3301
Abstract: Synaptotagmin‐like protein 4 (Slp4), expressed in human platelets, is associated with dense granule release. Slp4 is comprised of the N‐terminal zinc finger, Slp homology domain, and C2 domains. We synthesized a compact construct (the Slp4N… read more here.
Keywords: slp4n peptide; zinc; zinc finger; terminal zinc ... See more keywords
Purification and In Vitro Characterization of Zinc Finger Recombinases.
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DOI: 10.1007/978-1-4939-7169-5_15
Abstract: Zinc finger recombinases (ZFRs) are designer site-specific recombinases that have been adapted for a variety of genome editing purposes. Due to their modular nature, ZFRs can be customized for targeted sequence recognition and recombination. There… read more here.
Keywords: zinc finger; finger recombinases; purification vitro;
Engineered Zinc Finger DNA-Binding Domains: Synthesis, Assessment of DNA-Binding Affinity, and Direct Protein Delivery to Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7231-9_27
Abstract: Zinc finger proteins are the most common among families of DNA-binding transcription factors. Designer transcription factors generated by the fusion of engineered zinc finger DNA-binding domains (ZF-DBDs) to effector domains have been valuable tools for… read more here.
Keywords: zinc finger; delivery; dna; dna binding ... See more keywords
Stereotaxic Surgery and Viral Delivery of Zinc-Finger Epigenetic Editing Tools in Rodent Brain.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8799-3_17
Abstract: Delivery of engineered zinc-finger proteins (ZFPs) for targeted epigenetic remodeling in rodent brain can be facilitated by the use of viral vector-mediated gene transfer coupled with stereotaxic surgery techniques. Here we describe the surgical protocol… read more here.
Keywords: zinc finger; delivery; rodent brain; brain ... See more keywords