Analysis of Murein Hydrolases and Proteases Through Zymography.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1550-8_2
Abstract: Zymography has been used to analyze enzymatic activity and processing of enzymes for many years. We have used bacterial cells copolymerized into the acrylamide gel to analyze specific activity of murein hydrolases of interest. In… read more here.
Keywords: hydrolases proteases; proteases zymography; murein hydrolases; analysis murein ... See more keywords
Zymography Principles.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7111-4_1
Abstract: Zymography, the detection, identification, and even quantification of enzyme activity fractionated by gel electrophoresis, has received increasing attention in the last years, as revealed by the number of articles published. A number of enzymes are… read more here.
Keywords: zymography; zymography principles; biology;
Reverse Zymography: Overview and Pitfalls.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7111-4_11
Abstract: Reverse zymography is a technique by which protease inhibitor(s) in a sample could be electrophoretically separated in a substrate-impregnated acrylamide gel and their relative abundance could be semi-quantified. The gel after electrophoresis is incubated with… read more here.
Keywords: protease; overview pitfalls; reverse zymography; zymography overview ... See more keywords
Bonding to dentin using an experimental zirconium oxynitrate etchant.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of dentistry"
DOI: 10.1016/j.jdent.2021.103641
Abstract: OBJECTIVE To investigate, by means of microtensile bond strength test (µTBS), nanoleakage expression analysis (NL), gelatin zymography and in situ zymography, the effects of an experimental metal salt-based zirconium oxynitrate etchant [ZrO(NO3)2,] - ZON with… read more here.
Keywords: dentin; oxynitrate etchant; situ zymography; zirconium oxynitrate ... See more keywords
Gelatinolytic activity in dentin upon adhesive treatment
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DOI: 10.1038/s41598-024-78042-w
Abstract: In this multi-parameter study, the effect of diverse factors related to adhesive application on the activation of host-derived gelatinases was investigated by gelatin zymography, in-situ zymography, fluorogenic DQ-gelatin assay and micro-tensile bond-strength (μTBS) testing. Gelatin… read more here.
Keywords: zymography; upon adhesive; gelatinolytic activity; activity ... See more keywords
Quantification of the presence of enzymes in gelatin zymography using the Gini index
Sign Up to like & getrecommendations! Published in 2022 at "Journal of bioinformatics and computational biology"
DOI: 10.1142/s0219720022500251
Abstract: Gel zymography quantifies the activity of certain enzymes in tumor processes. These enzymes are widely used in medical diagnosis. In order to analyze them, experts classify the zymography spots into various classes according to their… read more here.
Keywords: enzymes gelatin; methodology; presence enzymes; gelatin zymography ... See more keywords
The Influence of Different Bleaching Protocols on Dentinal Enzymatic Activity: An In Vitro Study
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27051684
Abstract: This study aimed to investigate matrix metalloproteinase (MMP) activity in human dentin using in-situ and gelatin zymography, after at-home and in-office bleaching, related to their clinical exposure times. Dentin specimens (n = 5) were treated… read more here.
Keywords: dentinal enzymatic; carbamide peroxide; activity; zymography ... See more keywords