LAUSR

The present work focuses on studying the degradation of industrial synthetic dyes, which poses serious health hazards and a drastic impact on the environment. Currently available enzymatic processes have higher production and operational costs. However, most enzymes are active at acidic pH, which limits its application in textile dye degradation. This problem can be overcome by lignolytic enzymes obtained from halo-alkaliphile through Solid State Fermentation (SSF) using wheat bran (agro-byproduct) as a substrate. The major lignolytic enzymes studied were Lignin Peroxidase (LiP), Manganese Peroxidase (MnP), and laccase. The results demonstrated the highest activity of 215.4 ± 1.57 of LiP, 36.8 ± 2.38 of MnP, and 8.34 ± 0.21 IU/gds of laccase. Crude enzymes were used to treat synthetic dyes (mainly azo dyes), and their potential for its degradation was confirmed by spectrophotometric, GC-MS, and HPLC analysis. The highest decolorization of 82-93% of Malachite Green (MG) was achieved in LiP and MnP mediated reaction system within 2 hours. The laccase reaction system showed degradation of 53.87% of methyl orange without adding any redox mediator. After obtaining these results, the crude LiP and MnP in the reaction system were further subjected to decolorization at a higher MG concentration of 100-600 mg/L without a redox mediator. As a result, both LiP and MnP decolorized MG by 72-89%. Further, GC-MS analysis of MG biodegradation products confirmed the formation of less toxic low molecular weight products such as benzaldehyde and methanone.