Co-expression of an alcohol dehydrogenase and a cyclohexanone monooxygenase for cascade reactions facilitates the regeneration of the NADPH cofactor.
Sign Up to like & getrecommendations! Published in 2018 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2017.09.003
Abstract: The introduction of a three-enzyme cascade (comprising a cyclohexanone monooxygenase (CHMO), an alcohol dehydrogenase (ADH) and a lipase (CAL-A)) for the production of oligo-ε-caprolactone provided self-sufficiency with respect to NADPH-cofactor regeneration and reduced inhibiting effects… read more here.
Keywords: cyclohexanone monooxygenase; regeneration; expression; alcohol dehydrogenase ... See more keywords
Engineering Cyclohexanone Monooxygenase for the Production of Methyl Propanoate.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.6b00965
Abstract: A previous study showed that cyclohexanone monooxygenase from Acinetobacter calcoaceticus (AcCHMO) catalyzes the Baeyer-Villiger oxidation of 2-butanone, yielding ethyl acetate and methyl propanoate as products. Methyl propanoate is of industrial interest as a precursor of… read more here.
Keywords: methyl; methyl propanoate; acchmo; cyclohexanone monooxygenase ... See more keywords
In Vivo, High-Throughput Selection of Thermostable Cyclohexanone Monooxygenase (CHMO)
Sign Up to like & getrecommendations! Published in 2020 at "Catalysts"
DOI: 10.3390/catal10080935
Abstract: Cyclohexanone monooxygenase (CHMO) from Acinetobacter sp. NCIMB 9871 is characterized as having wide substrate versatility for the biooxidation of (cyclic) ketones into esters and lactones with high stereospecificity. Despite industrial potential, CHMO usage is restricted… read more here.
Keywords: cyclohexanone monooxygenase; cyclohexanone; selection; chmo ... See more keywords