A Fast and Easy Method for Specific Detection of Circular RNA by Rolling‐Circle Amplification
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201900514
Abstract: Circular RNAs (circRNAs) represent a new class of usually noncoding transcripts with largely unknown functions. Their research is hampered not least by the inapplicability of traditional analytical methods. Herein we describe a rapid and easy… read more here.
Keywords: rolling circle; detection; circle amplification; circular rna ... See more keywords
Non‐Invasive prenatal testing with rolling circle amplification: Real‐world clinical experience in a non‐molecular laboratory
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24870
Abstract: Non‐invasive prenatal testing (NIPT) using cell‐free DNA (cfDNA) circulating in maternal blood provides a sensitive and specific screening technique for common fetal aneuploidies, but the high cost and workflow complexity of conventional methodologies limit its… read more here.
Keywords: prenatal testing; rolling circle; non invasive; circle amplification ... See more keywords
Rolling circle amplification and graphene-based sensor-on-a-chip for sensitive detection of serum circulating miRNAs.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.04.016
Abstract: In this study, we developed a simple multiplex miRNA detection platform based on rolling circle amplification and the fluorescence quenching property of reduced graphene oxide. The detection platform could be applied on a microfluidics chip… read more here.
Keywords: rolling circle; detection; circle amplification; platform ... See more keywords
Ultrasensitive assay based on a combined cascade amplification by nicking-mediated rolling circle amplification and symmetric strand-displacement amplification.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.10.004
Abstract: MicroRNAs (miRNAs) play an important role in the regulation of various biological processes and have been used as potential biomarkers for biomedical research and clinical diagnosis. Here, nicking-mediated rolling circle amplification (N-RCA)/symmetric isothermal circular strand-displacement… read more here.
Keywords: strand displacement; rolling circle; nicking mediated; circle amplification ... See more keywords
Specific and simultaneous detection of micro RNA 21 and let-7a by rolling circle amplification combined with lateral flow strip.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.12.040
Abstract: Owing to the pivotal function in post transcriptional gene modification, miRNA biomarkers are playing crucial role in tracking and diagnosing various forms of tumors in a short time period. Hence, the need to develop simple,… read more here.
Keywords: detection; rolling circle; circle amplification; lateral flow ... See more keywords
Molecular inversion probe-rolling circle amplification with single-strand poly-T luminescent copper nanoclusters for fluorescent detection of single-nucleotide variant of SMN gene in diagnosis of spinal muscular atrophy.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.04.026
Abstract: In this study, a simple fluorescent detection of survival motor neuron gene (SMN) in diagnosis of spinal muscular atrophy (SMA) based on nucleic acid amplification test and the poly-T luminescent copper nanoclusters (CuNCs) was established.… read more here.
Keywords: detection; diagnosis; rolling circle; circle amplification ... See more keywords
Simple rolling circle amplification colorimetric assay based on pH for target DNA detection.
Sign Up to like & getrecommendations! Published in 2019 at "Talanta"
DOI: 10.1016/j.talanta.2019.04.003
Abstract: Detection and identification of DNA by PCR has opened tremendous possibilities and allows detection of minute quantities of DNA highly specifically. However, PCR remains confined to laboratory settings because of the need of thermocyclers and… read more here.
Keywords: dna; circle amplification; colorimetric assay; rolling circle ... See more keywords
Single Particle Inductively Coupled Plasma Mass Spectrometry-Based Homogeneous Detection of HBV DNA with Rolling Circle Amplification-Induced Gold Nanoparticle Agglomeration.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00272
Abstract: A highly sensitive and simple method based on rolling circle amplification (RCA) and single particle inductively coupled plasma mass spectrometry (spICP-MS) was proposed for the homogeneous detection of hepatitis B virus (HBV) deoxyribonucleic acid (DNA).… read more here.
Keywords: dna; rolling circle; hbv; circle amplification ... See more keywords
"Off" to "On" Surface-Enhanced Raman Spectroscopy Platform with Padlock Probe-Based Exponential Rolling Circle Amplification for Ultrasensitive Detection of MicroRNA 155.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.6b04082
Abstract: In this work, an "off" to "on" surface-enhanced Raman spectroscopy (SERS) platform was constructed for ultrasensitive detection of microRNA (miRNA) by using a magnetic SERS substrate (Co@C/PEI/Ag) and padlock probe-based exponential rolling circle amplification (P-ERCA)… read more here.
Keywords: detection; rolling circle; circle amplification; spectroscopy ... See more keywords
Versatile Electrochemiluminescence Assays for PEDV Antibody Based on Rolling Circle Amplification and Ru-DNA Nanotags.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b00853
Abstract: The sensitive and accurate detection methods for PEDV antibody have practical significance for the prevention and treatment of PEDV. In this work, a new multiple pathways signal amplification method was proposed to construct a sensitive… read more here.
Keywords: pedv antibody; rolling circle; circle amplification; antibody ... See more keywords
Highly Sensitive Genosensing Coupling Rolling Circle Amplification with Multiple DNAzyme Cores for DNA Walking.
Sign Up to like & getrecommendations! Published in 2020 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.9b00861
Abstract: Herein, a highly sensitive electrochemical genosensor is proposed by the construction of novel DNA walking machine. Generally, a number of tetrahedral DNA (TDNA) supported tracks and walkers are co-modified on the electrode surface. DNA walking… read more here.
Keywords: dna; rolling circle; circle amplification; highly sensitive ... See more keywords